- Evolutionary microbiologist
- Archaeal genomics, metabolism and evolution
- Microbial symbiosis and Eukaryogenesis
- Metagenomics, comparative genomics, phylogenomics, cultivation
Prof. Dr. Anja Spang
Archaea - a window into the history of life
Evolutionary microbiologist Dr Anja Spang investigates archaea. ‘These are the single-celled microorganisms which form one of two primary domains – the other being Bacteria. Initially, the Archaea were mainly known for their ability to survive in extreme environments. But it has since become clear that they occur almost everywhere on Earth. They are not only found on our bodies, but also in soils, lakes, the ocean and in sediments.
The basis of all “higher” life
‘Although the Archaea form a primary branch in the tree of life just like Bacteria, they are much less studied so far. For example, many details regarding their role in natural ecosystems and in the evolution of life remain unknown. I have contributed to recent research which suggests that the complex eukaryotic cells - which comprise organisms such as algae, protists, plants, fungi and animals - originated from a symbiosis between archaea and bacteria.’
Evolution of archaea in marine ecosystems
With my research team at NIOZ, we now aim to further illuminate the role of Archaea in life’s evolution and elucidate how symbiotic interactions have shaped microbial diversity. Furthermore, we are interested in understanding how symbiotic archaea contribute to the structure of microbial communities, especially in the poorly investigated marine environments such as the deep waters, sediments and hydrothermal vents. The DPANN archaea are particularly interesting in this context because they comprise a very diverse group of symbiotic archaea, which may may have played an important role in the evolution of life. Furthermore, DPANN, just like viruses, need a host organism for growth and may play important roles in microbial food webs.
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Research interests
I have always been fascinated by the evolution of life on Earth, eukaryogenesis and the role symbiosis has played in the major transitions of life. My past research on Archaea has been driven by the aim of getting a better understanding of the diversity and genomic potential of Archaea and their relationship to Bacteria and eukaryotes. For example, during the past years, my post-doctoral research in the lab of Thijs Ettema at Uppsala University (see links below), focused on the investigation of Lokiarchaeota (Spang et al., 2015) and related lineages, which together comprise the Asgard superphylum and have proven to be key for our understanding of the eukaryotic cell (Zaremba-Niedzwiedzka et al., 2017). We could show that Asgard archaea form a monophyletic group with Eukaryotes and encode various eukaryotic signature proteins, suggesting that they have been important in the early stages of the origin of the eukaryotic cell (Spang et al., 2015, Zaremba et al., 2017, Spang et al., 2017, Eme et al., 2017). The recent investigation of the metabolic potential of the Asgard archaea in collaboration with the Ettema lab, has recently led us to propose an updated scenario on the origin of the eukaryotic cell - the reverse syntrophy hypothesis - from a symbiosis between an hydrogen or electron producing Asgard-archaeal ancestor and (a) bacterial partner(s) (Spang et al., 2019).
With my research team at NIOZ, we aim to further illuminate the role of Archaea in the evolution of life on Earth and to elucidate the extent to which syntrophic interactions and symbioses have enabled major transitions in the Tree of life (ToL) and contribute to the structure and evolution of microbial communities.
My research aligns with the new science plan of NIOZ, which addresses pressing questions concerning the past and future functioning of seas and oceans. In particular, as an evolutionary microbiologist, I have a key interest in contributing to a better understanding of the history of life on Earth as a mean to better constrain the future evolution of life in an ever-changing world. A major focus of my research is to establish the role of archaeal and bacterial symbionts in the earliest evolution of cells, the subsequent diversification of these cells that led to the astounding diversity of microbial life on Earth as well as their role in the origin of eukaryotes. Furthermore, I am studying the impact of extant symbionts on the diversification of life in an ever-changing world, their impact on host ecology and evolution and their importance in extant nutrient cycles. For example, it has recently been suggested that archaeal symbionts belonging to the DPANN archaea play antagonistic roles in food webs similar to viruses and phages. Yet, in contrast to the well-established role of viruses in microbial food webs, DPANN symbionts have so far not been taken into account in ecological models. To obtain a better understanding of the functioning of marine food webs, it will be crucial to determine who are the hosts of the extremely diverse DPANN archaea in marine waters and sediments as well as elucidate their impact on host ecology and evolution and organic matter recycling. Besides my major focus on Archaea, I am also involved in or driving projects on the evolution of Bacteria and Eukaryotes. For example, in the frame of a UU-NIOZ collaboration, we study the diversification of eukaryotic metabolisms in light of Earth history as a basis to make predictions on the future evolution of eukaryotic life in changing marine environments.
Team
This research would not be possible without my great team, currently including the following talented scientists:
- post-docs
Joshua Hamm: https://www.nioz.nl/en/about/organisation/staff/joshua-hamm
Oleksandr Maistrenko: https://www.nioz.nl/en/about/organisation/staff/oleksandr-maistrenko
Florian Mayer: https://www.nioz.nl/en/about/organisation/staff/florian-mayer
Carlos Santana Molina: https://www.nioz.nl/en/about/organisation/staff/carlos-santana-molina
Dina Castillo Boukhchtaber: https://www.nioz.nl/en/about/organisation/staff/dina-castillo-boukhchtaber
- Phd student
Tara Mahendrarajah: https://www.nioz.nl/en/about/organisation/staff/tara-mahendrarajah
Wen-Cong Huang: https://www.nioz.nl/en/about/organisation/staff/wen-cong-huang
- Previous members
- Nina Dombrowski (2018-2023)
- Scott Maxson, technical assistant (2021-2023)
- Gerben de Zwaan (master student, 2022)
- Josje Romeijn (master student, 2022)
- Kim van Maldegem (master student, 2021)
- Jun-Hoe Lee (post-doc, 2017-2019)
Education & research experience
Since 12/2022: Special Chair Professor in 'Symbioses in Evolution' at the University of Amsterdam
Since 11/2022: Research Leader at NIOZ, Netherlands Institute for Sea Research (80%)
1/2021-10/2022: Senior Scientist at NIOZ, Netherlands Institute for Sea Research (100%)
9/2017-12/2021: Tenure track researcher at NIOZ, Netherlands Institute for Sea Research (80%) (tenured Senior Scientist from 3/2021) & VR-funded researcher at Uppsala University, Department of Cell- and Molecular Biology (20%)
5/2013-8/2017: Postdoctoral researcher at the Department of Cell- and Molecular Biology, Science for Life Laboratory, Uppsala University, Sweden; Group Leader: Dr. Thijs Ettema
Main topics: Archaeal roots of Eukaryotes, Comparative genomics of (Asgard) archaea
1/2009-5/2013: PhD studies in microbial comparative genomics in the Department of Genetics in Ecology at the University of Vienna (Austria)
Topic: Genome Analyses and Comparative Genomics of Thaumarchaeota
2007-2008: Master studies in microbial genomics, University of Bergen (Norway); Topic: Metagenomics of archaeal viruses from Icelandic hot springs
Grants/Fellowships
2023-2026: NWO M-grant for aquatic symbioses
05/2023: Ammodo Science Award for fundamental research
2021-2026: ERC starting grant (947317, ASymbEL) to study to address the role of Archaeal Symbionts in the Evolution of Life.
2021-2024: UU-NIOZ award.
2020-2023: Symbiosis Model System Award from the Gordon and Betty Moore Foundation.
2020-2023: Eukaryogenesis Award from the Gordon and Betty Moore Foundation.
2018-2021: NWO Women In Science Excel (WISE) tenure track award providing five years of funding for establishing an independent research group
2017-2021: VR starting grant from the Swedish Research council (Vetenskapsrådet) (duration: four years)
2013-2016: Marie-Curie Intra-European Fellowship by the European Commission (duration: two years, starting date autumn 2014)
2010-2012: Two years of research funded by a DOC-fFORTE fellowship from the Austrian Academy of Sciences
Publications:
Contact me:
email: anja.spang@nioz.nl or a.spang@uva.nl
twitter: anjspa1
Linked news
NIOZ publications
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2024
Williams, T.A.; Davín, A.A.; Szánthó, L.L.; Stamatakis, A.; Wahl, N.A.; Woodcroft, B.J.; Soo, R.M.; Eme, L.; Sheridan, P.; Gubry-Rangin, C.; Spang, A.; Hugenholtz, P.; Szöllosi, G.J. (2024). Phylogenetic reconciliation: making the most of genomes to understand microbial ecology and evolution. ISME J. 18(1): wrae129. https://dx.doi.org/10.1093/ismejo/wrae129Moody, E.R.R.; Álvarez-Carretero, S.; Mahendrarajah, T.A.; Clark, J.W.; Betts, H.C.; Dombrowski, N.; Szánthó, L.L.; Boyle, R.A.; Daines, S.; Chen, X.; Lane, N.; Yang, Z.; Shields, G.A.; Szöllosi, G.J.; Spang, A.; Pisani, D.; Williams, T.A.; Lenton, T.M.; Donoghue, P.C.J. (2024). The nature of the last universal common ancestor and its impact on the early Earth system. Nature Ecology & Evolution 8(9): 1654-1666. https://dx.doi.org/10.1038/s41559-024-02461-1Hamm, J.N.; Liao, Y.; von Kügelgen, A.; Dombrowski, N.; Landers, E.; Brownlee, C.; Johansson, E.M.V.; Whan, R.M.; Baker, M.; Baum, B.; Bharat, T.A.M.; Duggin, I.G.; Spang, A.; Cavicchioli, R. (2024). The parasitic lifestyle of an archaeal symbiont. Nature Comm. 15(1): 6449. https://dx.doi.org/10.1038/s41467-024-49962-yGreening, C.; Cabotaje, P.R.; Valentin A., L.E.; Leung, P.M.; Land, H.; Rodrigues-Oliveira, T.; Ponce-Toledo, R.I.; Senger, M.; Klamke, M.A.; Milton, M.; Lappan, R.; Mullen, S.; West-Roberts, J.; Mao, J.; Song, J.; Schoelmerich, M.; Stairs, C.W.; Schleper, C.; Grinter, R.; Spang, A.; Banfield, J.F.; Berggren, G. (2024). Minimal and hybrid hydrogenases are active from archaea. Cell 187(13): 3357-3372.e19. https://dx.doi.org/10.1016/j.cell.2024.05.032
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2023
Mahendrarajah, T.A.; Moody, E.R.R.; Schrempf, D.; Szánthó, L.L.; Dombrowski, N.; Davín, A.A.; Pisani, D.; Donoghue, P.C.J.; Szöllosi, G.J.; Williams, T.A.; Spang, A. (2023). ATP synthase evolution on a cross-braced dated tree of life. Nature Comm. 14(1): 7456. https://dx.doi.org/10.1038/s41467-023-42924-wDonoghue, P.C.J.; Kay, C.; Spang, A.; Szöllosi, G.J.; Nenarokova, A.; Moody, E.R.R.; Pisani, D.; Williams, T.A. (2023). Defining eukaryotes to dissect eukaryogenesis. Curr. Biol. 33(17): R919-R929. https://dx.doi.org/10.1016/j.cub.2023.07.048Williams, T.A.; Davín, A.A.; Morel, B.; Szánthó, L.L.; Spang, A.; Stamatakis, A.; Hugenholtz, P.; Szöllosi, G.J. (2023). Parameter Estimation and Species Tree Rooting Using ALE and GeneRax. Genome Biology and Evolution 15(7). https://dx.doi.org/10.1093/gbe/evad134Spang, A. (2023). Uncovering the hidden world of nanosized archaea. Nat. Rev., Microbiol. 21(10): 638-638. https://dx.doi.org/10.1038/s41579-023-00912-3van der Gulik, P.T.S.; Egas, M.; Kraaijeveld, K.; Dombrowski, N.; Groot, A.T.; Spang, A.; Hoff, W.D.; Gallie, J. (2023). On distinguishing between canonical tRNA genes and tRNA gene fragments in prokaryotes. Rna Biology 20: 48-58. https://dx.doi.org/10.1080/15476286.2023.2172370
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2022
Albers, S.; Ashmore, J.; Pollard, T.; Spang, A.; Zhou, J. (2022). Origin of eukaryotes: What can be learned from the first successfully isolated Asgard archaeon. Faculty reviews 11. https://dx.doi.org/10.12703/r-01-000005Spang, A. (2023). Is an archaeon the ancestor of eukaryotes? Environ. Microbiol. 25(4): 775-779. https://dx.doi.org/10.1111/1462-2920.16323Spang, A.; Mahendrarajah, T.A.; Offre, P.; Stairs, C.W. (2022). Evolving perspective on the origin and diversification of cellular life and the virosphere. Genome Biology and Evolution 14(6): evac034. https://dx.doi.org/10.1093/gbe/evac034Krause, S.; Gfrerer, S.; von Kügelgen, A.; Reuse, C.; Dombrowski, N.; Villanueva, L.; Bunk, B.; Spröer, C.; Neu, T.R.; Kuhlicke, U.; Schmidt-Hohagen, K.; Hiller, K.; Bharat, T.A.M.; Rachel, R.; Spang, A.; Gescher, J. (2022). The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host. Nature Comm. 13: 1735. https://dx.doi.org/10.1038/s41467-022-29263-yPatterson, M.O.; Levy, R.H.; Kulhanek, D.K.; van de Flierdt, T.; Horgan, H.; Dunbar, G.B.; Naish, T.R.; Ash, J.; Pyne, A.; Mandeno, D.; Winberry, P.; Harwood, D.M.; Florindo, F.; Jimenez-Espejo, F.J.; Läufer, A.; Yoo, K.-C.; Seki, O.; Stocchi, P.; Klages, J.P.; Lee, J.I.; Colleoni, F.; Suganuma, Y.; Gasson, E.; Ohneiser, C.; Flores, J.-A.; Try, D.; Kirkman, R.; Koch, D.; the SWAIS 2C Science Team (2022). Sensitivity of the West Antarctic Ice Sheet to +2 °C (SWAIS 2C). Sci. Drill. 30: 101-112. https://dx.doi.org/10.5194/sd-30-101-2022Moody, E.R.R.; Mahendrarajah, T.A.; Dombrowski, N.; Clark, J.W.; Petitjean, C.; Offre, P.; Szöllosi, G.J.; Spang, A.; Williams, T.A. (2022). An estimate of the deepest branches of the tree of life from ancient vertically evolving genes. eLIFE 11: e66695. https://dx.doi.org/10.7554/elife.66695
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2021
Dombrowski, N.; Mahendrarajah, T.A.; Gross, S.T.; Eme, L.; Spang, A. (2021). Archaea, in: Green, L.H. et al. Practical Handbook of Microbiology, 4th edition. pp. 229-248. https://doi.org/10.1201/9781003099277-23Coleman, G.A.; Davín, A.A.; Mahendrarajah, T.A.; Szánthó, L.L.; Spang, A.; Hugenholtz, P.; Szöllosi, G.J.; Williams, T.A. (2021). A rooted phylogeny resolves early bacterial evolution. Science (Wash.) 372(6542): eabe0511. https://doi.org/10.1126/science.abe0511
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2020
Reysenbach, A.-L.; St. John, E.; Meneghin, J.; Flores, G.E.; Podar, M.; Dombrowski, N.; Spang, A.; L'Haridon, S.; Humphris, S.E.; De Ronde, C.E.J.; Caratori-Tontini, F.; Tivey, M.; Stucker, V.K.; Stewart, L.C.; Diehl, A.; Bach, W. (2020). Complex subsurface hydrothermal fluid mixing at a submarine arc volcano supports distinct and highly diverse microbial communities. Proc. Natl. Acad. Sci. U.S.A. 117(51): 32627-32638. https://doi.org/10.1073/pnas.2019021117Martijn, J.; Schön, M.E.; Lind, A.E.; Vosseberg, J.; Williams, T.A.; Spang, A.; Ettema, T.J.G. (2020). Hikarchaeia demonstrate an intermediate stage in the methanogen-to-halophile transition. Nature Comm. 11: 5490. https://doi.org/10.1038/s41467-020-19200-2Stairs, C.W.; Sharamshi, J.E.; Tamarit, D.; Eme, L.; Jorgensen, S.L.; Spang, A.; Ettema, T.J.G. (2020). Chlamydial contribution to anaerobic metabolism during eukaryotic evolution. Science Advances 6(35): eabb7258. https://dx.doi.org/10.1126/sciadv.abb7258Dombrowski, N.; Williams, T.A.; Sun, J.; Woodcroft, B.J.; Lee, J.-H.; Minh, B.Q.; Rinke, C.; Spang, A. (2020). Undinarchaeota illuminate DPANN phylogeny and the impact of gene transfer on archaeal evolution. Nature Comm. 11: Article number: 3939. https://dx.doi.org/10.1038/s41467-020-17408-wMurray, A. E.; Freudenstein, J.; Gribaldo, S.; Hatzenpichler, R.; Hugenholtz, P.; Kämpfer, P.; Konstantinidis, K. T.; Lane, C. E.; Papke, R. T.; Parks, D. H.; Rossello-Mora, R.; Stott, M. B.; Sutcliffe, I. C.; Thrash, J. C.; Venter, S. N.; Whitman, W. B.; Acinas, S. G.; Amann, R. I.; Anantharaman, K.; Armengaud, J.; Baker, B. J.; Barco, R. A.; Bode, H. B.; Boyd, E. S.; Brady, C. L.; Carini, P.; Chain, P. S. G.; Colman, D. R.; DeAngelis, K. M.; de los Rios, Ma. A.; Estrada-de los Santos, P.; Dunlap, C. A.; Eisen, J. A.; Emerson, D.; Ettema, T. J. G.; Eveillard, D.; Girguis, P. R.; Hentschel, U.; Hollibaugh, J. T.; Hug, L. A.; Inskeep, W. P.; Ivanova, E. P.; Klenk, H.-P.; Li, W.-J.; Lloyd, K. G.; Löffler, F. E.; Makhalanyane, T. P.; Moser, D. P.; Nunoura, T.; Palmer, M.; Parro, V.; Pedrós-Alió, C.; Probst, A. J.; Smits, T. H. M.; Steen, A. D.; Steenkamp, E. T.; Spang, A.; Stewart, F. J.; Tiedje, J. M.; Vandamme, P.; Wagner, M.; Wang, F.-P.; Hedlund, B. P.; Reysenbach, A.-L. (2020). Roadmap for naming uncultivated Archaea and Bacteria. Nature Microbiology 5(8): 987-994. https://dx.doi.org/10.1038/s41564-020-0733-xDharamshi, J.E.; Tamarit, D.; Eme, L.; Stairs, C.W.; Martijn, J.; Homa, F.; Jorgensen, S.L.; Spang, A.; Ettema, T.J.G. (2020). Marine sediments illuminate Chlamydiae diversity and evolution. Curr. Biol. 30(6): 1032-1048.e7. https://dx.doi.org/10.1016/j.cub.2020.02.016
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2019
Blohs, M.; Mahnert, A.; Spang, A.; Dombrowski, N.; Krupovica, M.; Klingl, A. (2019). Archaea – An Introduction, in: Schmidt, R.M. Encyclopedia of Microbiology. pp. 243-252. https://dx.doi.org/10.1016/b978-0-12-809633-8.20884-4Camprubí, E.; de Leeuw, J.W.; House, C.H.; Raulin, F.; Russell, M.J.; Spang, A.; Tirumalai, M.R.; Westall, F. (2019). The emergence of life. Space Science Reviews 215(8). https://dx.doi.org/10.1007/s11214-019-0624-8Schwank, K.; Bornemann, T.L.V.; Dombrowski, N.; Spang, A.; Banfield, J.F.; Probst, A. (2019). An archaeal symbiont-host association from the deep terrestrial subsurface. ISME J. 13(8): 2135-2139. https://dx.doi.org/10.1038/s41396-019-0421-0Seitz, K.W.; Dombrowski, N.; Eme, L.; Spang, A.; Lombard, J.; Sieber, J.R.; Teske, A.P.; Ettema, T.J.G.; Baker, B.J. (2019). Asgard archaea capable of anaerobic hydrocarbon cycling. Nature Comm. 10(1): 1822. https://dx.doi.org/10.1038/s41467-019-09364-xBäckström, D.; Yutin, N.; Jorgensen, S.L.; Dharamshi, J.; Homa, F.; Zaremba-Niedwiedzka, K.; Spang, A.; Wolf, Y.I.; Koonin, E.V.; Ettema, T.J.G. (2019). Virus genomes from deep sea sediments expand the ocean megavirome and support independent origins of viral gigantism. Mbio 10(2): e02497-18. https://dx.doi.org/10.1128/mbio.02497-18Spang, A.; Stairs, C.W.; Dombrowski, N.; Eme, L.; Lombard, J.; Caceres, E.F.; Greening, C.; Baker, B.J.; Ettema, T.J.G. (2019). Proposal of the reverse flow model for the origin of the eukaryotic cell based on comparative analyses of Asgard archaeal metabolism. Nature Microbiology 4: 1138–1148. https://dx.doi.org/10.1038/s41564-019-0406-9Dombrowski, N.; Lee, J.-H.; Williams, T.A.; Offre, P.; Spang, A. (2019). Genomic diversity, lifestyles and evolutionary origins of DPANN archaea. FEMS Microbiol. Lett. 366(2): 1-12. https://dx.doi.org/10.1093/femsle/fnz008Spang, A.; Offre, P. (2019). Towards a systematic understanding of differences between archaeal and bacterial diversity. Environmental Microbiology Reports 11(1): 9-12. https://dx.doi.org/10.1111/1758-2229.12701
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2018
Kellner, S.; Spang, A.; Offre, P.; Szöllosi; Petitjean, C.; Williams, T.A. (2018). Genome size evolution in the Archaea. Emerging Topics in Life Sciences 2(4): ETLS20180021. https://dx.doi.org/10.1042/etls20180021Narrowe, A.B.; Spang, A.; Stairs, C.W.; Caceres, E.F.; Baker, B.J.; Miller, C.S.; Ettema, T.J.G. (2018). Complex evolutionary history of translation elongation factor 2 and diphthamide biosynthesis in archaea and parabasalids. Genome Biology and Evolution 10(9): 2380-2393. https://doi.org/10.1093/gbe/evy154Raina, J.-B.; Eme, L.; Pollock, F.J.; Spang, A.; Archibald, J.M.; Williams, T.A. (2018). Symbiosis in the microbial world: from ecology to genome evolution. Biology Open 7(2): bio032524. https://doi.org/10.1242/bio.032524
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