Royal Netherlands Institute for Sea Research
Royal Netherlands
Institute for Sea Research
Phone number
+31 (0)222 369 489
Tenure track Scientist
  • - Physiology and metabolism of microorganisms
  • - Ecology of microorganisms
  • - Archaea and Bacteria
  • - Biogeochemical cycling of carbon and nitrogen

Dr. Pierre Offre

Tenure track Scientist

Research interests

The astounding diversity of metabolisms, physiologies and ecological strategies in both extent and past life forms is at the center of my motivation for biological sciences. I have a particular interest in microorganisms, especially Bacteria and Archaea, because of the incredible variety of their primary and secondary metabolisms, which fascinates me. The variety of those metabolisms is key to the (re)cycling of nutrients essential to all life forms and is therefore a central component of the biogeochemical engine that enabled life to thrive and diversify on Earth for up to 4 billion years. The overall aim of my research is to characterize metabolic and physiological traits of microorganisms, determine their molecular basis and understand how those traits drive the cycling of nutrients in ecosystems and the assembly of biological communities. I address those overarching goals in two distinct lines of research:

Ecological niche and life strategies of archaeal ammonia oxidizers in dark ocean waters: The ammonia-oxidizing archaea (AOA) are chemo-litho-autotrophic aerobes that conserve energy and produce reducing equivalents from the oxidation of ammonia into nitrite. Those microorganisms are thought to play a major role in the ocean nitrogen cycle and uncultivated relatives of those microorganisms account for up to 40% of all bacteria and archaea in dark ocean waters. The large proportion of those putative autotrophs in deep waters challenges the idea that most deep-sea microorganisms are heterotrophs depending on the vertical flux of particulate organic matter. Those observations challenge as well our knowledge of the lifestyle of AOA and their deep-water relatives and question our understanding of nitrogen cycling in the ocean. Using a combination of physiological experiments on cultivated AOA strains, enrichment cultures, isotopic tracer studies and ‘omics approaches, my team investigates the physiological and metabolic traits enabling relatives of cultivated AOA to thrive in the deep ocean and clarify models of deep-water nitrogen cycling.

Metabolic and physiological diversity of JTB255 bacteria: JTB255 is a group of Gammaproteobacteria, which was recently shown to be widely distributed in marine benthic environments, including both coastal and deep-sea sediments. Sequencing surveys suggest that JTB255 could account for a large fraction of all bacteria and archaea in sediments and may indeed represent one of the core groups of the global sediment microbiome. Until recently JTB255 was only known as a cluster of 16S rRNA gene sequences but several partial genomes and one complete genome have now been obtained. Investigations of those genomes suggested that JTB255 bacteria include some chemo-organo-heterotrophs both strict and facultative (some have a potential for hydrogen and sulfur-based chemo-litho-autotrophy). My research team aims at characterizing the metabolic and physiological diversity of those bacteria using growth experiments on a cultivated strain, enrichment cultures and ‘omics investigations in order to further our knowledge of the ecological role of JTB255.

Research Experience

Since 09/2017: Tenure Track Scientist at NIOZ, Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry (MMB)

04/2015 – 09/2017:  Senior Scientist at Max-Planck Institute for Marine Microbiology (Germany), HGF MPG Joint Research Group for Deep-Sea Ecology and Technology

01/2015 – 03/2015: Visiting Scientist at University of Uppsala (Sweden), Department of Limnology

08/2010 – 12/2014:  Postdoctoral Researcher at University of Vienna (Austria), Department of Ecogenomics and Systems Biology

05/2008 – 08/2010:  Postdoctoral Researcher at University of Aberdeen (United Kingdom), Institute of Biological and Environmental Sciences

01/2008 – 04/2008:  Researcher at INRA-Dijon (France), Department ‘Plants, Microbes and Environments’

12/2003 – 06/2007:  PhD student at INRA-Dijon (France), Department ‘Soil and Environmental Microbiology’

09/2002 – 07/2003:  Master student at INRA-Dijon (France), Department ‘Soil and Environmental Microbiology’


Google Scholar:

Linked news

Thursday 13 February 2020
A real global player: Previously unrecognised bacteria may be a key group in marine sediments
From the shoreline to the deep sea, one group of bacteria is particularly widespread in our planet’s seabed: The so-called Woeseiales, which may be feeding on the protein remnants of dead cells. Researchers from the Max Planck Institute for Marine…

Linked blogs

Tuesday 21 August 2018
NIOZ@SEA | Black Sea cruise 2018
The Black Sea is the largest permanently stratified low oxygen basin in the world and its water column is characterized by the presence of multiple redox gradients. This is an ideal setting to determine the physiology and role of microbes that do not…

NIOZ publications