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My project deals with the development of new lipid biomarker proxies for an-/aerobic methane oxidizers in present and past environments within the Netherlands Earth System Science Center (NESSC). A better understanding of the the sources, sinks and chemical reaction pathways in greenhouse gas cycling especially methane is important to constrain its impact on global climate changes. However, reliable biomarker tools for determining past methane concentrations in the atmosphere and the intensity of methane cycling are currently lacking.
In this project, I´m using mutiple analyzing techniques like: GC, GC-MS, GC-MS-MS, GC-IRMS, UHPLC-HRMS and molecular techniques like 16S rRNA seq. We investigate diverse lipid biomarkers and their 13C signatures as proxies for gas oxidation processes: fatty acids, mycocerosic acids, hopanoids , n-alkanes, GDGTs and petroleum biomarker
Education
10/2013 - 05/2016
Master of Marine Geosciences at the University of Bremen, Germany
Focus: Biogeochemistry, Geophysics and Marine Resources
Master thesis: Geochemical characterization of asphalt deposits in the Campeche Bay (southern Gulf of Mexico) - Insights in the persistence of heavy oil in the marine environment
10/2010 – 09/2013
Bachelor of Geosciences at the University of Bremen, Germany
Focus: Geochemistry, Marine Geology and Geodynamics
Bachelor thesis: Evaluation of the TEX86- paleotemperature proxy by archaeen cultures
Work experience
12/2011-03/2016
Student Assistant in the research group “Organic Geochemistry” MARUM, University of Bremen, Germany
11/2015
Internship in the framework of the Master thesis at Shell Global Solutions, Rijswijk, Netherlands
04/2105-07/2015
Research project in the research group “Organic Paleobiogeochemistry” MARUM and MPI, University of Bremen, Germany
Awards and scholarships
09/2019
Best Student Presentation Award at IMOG 2019, Gothenburg
04/2019
BASIS (Benelux Association for Stable Isotope Scientists) Presentation Award
09/2018
Scholarship NESSC (Netherlands Earth System Science Centre) exchange program
05/2017
MARUM Research Award for Marine Science 2017 for master thesis - Geochemical characterization of asphalt deposits in the Campeche Bay (southern Gulf of Mexico)
11/2016
EAOG (European Association of Organic Geochemists) Travel Scholarship Award 2016
References
Witkowski, C.R., van der Meer, M.T., Smit, N.T., Damsté, J.S.S., Schouten, S., 2020. Testing algal-based p CO 2 proxies at a modern CO 2 seep (Vulcano, Italy). Scientific RepoRtS 10, 1-10.
Smit, N.T., Rush, D., Sahonero-Canavesi, D.X., Verweij, M., Rasigraf, O., Cruz, S.G., Jetten, M.S., Damsté, J.S.S., Schouten, S., 2019b. Demethylated hopanoids in ‘Ca. Methylomirabilis oxyfera’as biomarkers for environmental nitrite-dependent methane oxidation. Organic Geochemistry 137, 103899.
Kurth, J.M., Smit, N.T., Berger, S., Schouten, S., Jetten, M.S., Welte, C.U., 2019b. Anaerobic methanotrophic archaea of the ANME-2d clade feature lipid composition that differs from other ANME archaea. FEMS microbiology ecology 95, fiz082.
Kurth, J.M., Smit, N.T., Berger, S., Schouten, S., Jetten, M.M., Welte, C.U., 2019a. ANME-2d anaerobic methanotrophic archaea differ from other ANME archaea in lipid composition and carbon source. bioRxiv, 558007.
Laso-Pérez, R., Hahn, C., van Vliet, D.M., Tegetmeyer, H.E., Schubotz, F., Smit, N.T., Pape, T., Sahling, H., Bohrmann, G., Boetius, A., 2019. Anaerobic degradation of non-methane alkanes by “Candidatus Methanoliparia” in hydrocarbon seeps of the Gulf of Mexico. MBio 10, e01814-01819.
Smit, N., Rush, D., Van Der Meer, M., Grassa, F., Villlanueva, L., Sinninghe Damsté, J., Schouten, S., Year. Proceedings of 29th International Meeting on Organic Geochemistry, IMOG 2019.
Smit, N., Grassa, F., Rush, D., Hopmans, E.C., Sinninghe Damsté, J.S., Schouten, S., 2018. Aerobic methane oxidation in soils as revealed by lipid biomarkers at terrestrial methane seeps in southern Sicily, Italy. EGUGA, 14480.
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