Dr. Corina P.D. Brussaard Senior Scientist	ROYAL NETHERLANDS INSTITUTE FOR SEA RESEARCH   Postal address: P.O. Box 59, NL-1790 AB Den Burg (Texel) The Netherlands   Visiting address: Landsdiep 4 NL-1797 SZ ’t Horntje (Texel) The Netherlands   Phone: (+31) (0)222-369300 Fax: (+31) (0)222-319674
E-mail: corina.brussaard@nioz.nl   Phone: (+31) (0)222-369513
Research Interests
Marine Viral Ecology. Current research in the lab involves:   Studying temporal and spatial dynamics of marine viruses and their hosts. Investigating the functional relationship of specific viruses in relation to their algal host. Determining the mortality rates of phytoplankton and bacteria due to viral infection. Comparing the ecological importance of viral lysis to other loss factors (e.g. microzooplankton grazing). Studying the impact of viral lysis on the structure and function of the microbial food web. Isolation and characterization of novel marine viruses, including full genome sequencing. Developing molecular approaches for viral diversity studies. ·         Studying under controlled conditions the interactions between virus and host in relation to environmental relevant factors.
Current Funded Projects
Virus induced mortality of marine phytoplankton in ecosystems with contrasting trophic status (MOMAP, ALW-NWO) PI: Dr. C.P.D. Brussaard PhD-student in project: A-C. Baudoux   Virus control of the picophytoplankter Micromonas pusilla population dynamics in European waters (MICROVIR, ALW-NWO) PI: Dr. C.P.D. Brussaard Post Doc in project: Dr. J. Martinez Martinez   The significance of viruses for polar marine ecosystem functioning (VIRPOL, IPY-NL). PI: Dr. C.P.D. Brussaard Post Doc in project: Dr. C. Evans   Fast Advanced Cellular and Ecosystems Information Technologies (FACEiT, 6th FWP EU). PI: Dr. C.P.D. Brussaard Post Doc in project: Dr. L. Peperzak   Comparative genomic analysis of viruses infecting Phaeocystis globosa and Micromonas pusilla, two eukaryotic microalga of global distribution. (US-DOE JGI). PI: Dr. C.P.D. Brussaard Co-PI: Dr. E. Wommack   Whole genome sequencing of a Phaeocystis globosa virus. (FR-GENOSCOPE). PI: Prof. JM. Claverie Co-PI: Dr. C. Brussaard
Other Topics
Finalized abstracts - 4th Algal Virus Workshop   Amsterdam, The Netherlands (17-21 April 2005)
Student Opportunities
We are regularly seeking for interns (stageplaats) and University students (Master topic). Skills recommended: microbial ecology and/or marine biology and/or virology and/or molecular techniques. Do not hesitate to contact me (corina.brussaard@nioz.nl) for further information.
Publication List (last 5 years)
Brussaard CPD, Bratbak, Baudoux A-C, Ruardij P. In press. Phaeocystis and its interaction with viruses. Biogeochemistry, special volume. Brussaard CPD. In press. Enumeration of bacteriophage using flow cytometry. Chapter 10 in Bacteriophages: Methods and Protocols. Eds. M Clokie and A Kropinski. Verity PG, Brussaard CPD, Njesgaard JC, Van Leeuwe MA, Lancelot C, Medlin LK. 2007. Current understanding of Phaeocystis ecology and biogeochemistry, and perspectives for future research. Biogeochemistry. Timmermans K, Veldhuis MJW, Brussaard CPD. 2007. The influence of light, silicate and iron limitation on algal mortality. Aquat. Micr. Ecol. 46:253-261. Lawrence J, Brussaard CPD Suttle CA. 2006. Virus-specific responses of Heterosigma akashiwo to infection. Appl. Environ. Microbiol. 72: 7829-7834. Baudoux AC, Noordeloos AAM, Veldhuis MJW, Brussaard CPD. 2006. Virally induced mortality of Phaeocystis globosa during two spring blooms in temperate coastal waters. Aquat. Microb. Ecol. 44: 207-217. Veldhuis MJW, Brussaard CPD. Harmful algae and cell death. 2006. Chapter 12: 153-162, in Ecological Studies, Vol. 89, Ecology of Harmful Algae. Eds. E. Granéli and J.T. Turner. Attoui H, Mohd Jaafar F, Belhouchet M, de Micco P, de Lamballerie X, Brussaard CPD. 2006. Micromonas pusila reovirus: a new member of the family Reoviridae assigned to a novel proposed genus (Mimoreovirus). J. Gen. Virology 87: 1375-1383. Franklin DJ, Brussaard CPD, Berges JA. 2006. Review: What is the role and nature of programmed cell death in phytoplankton ecology? Eur. J. Phycol. 41: 1-14. Baudoux AC, Noordeloos AAM, Brussaard CPD. 2005. Characterization of different viruses infecting Phaeocystis globosa. Virology 341: 80-90. Brussaard CPD, Kuipers B, Veldhuis MJW. 2005. A mesocosms study of Phaeocystis globosa population dynamics. I. Regulatory role of viruses in bloom control. Harmful Algae 4: 859-874. Brussaard CPD, Mari X, Van Bleijswijk JDL, Veldhuis MJW. 2005. A mesocosm study of Phaeocystis globosa population dynamics. II. Signficance for the microbial community. Harmful Algae 4: 875-893. Ruardij P, Veldhuis MJW, Brussaard CPD. 2005. Modelling the development and termination of a Phaeocystis bloom. Harmful Algae 4: 941-963. Veldhuis MJW, Brussaard CPD, Noordeloos AAM. 2005. Living in a Phaeocystis colony; a way to be a successful algal species. Harmful Algae 4: 841-858. Mari X. Rassoulzadegan F, Brussaard CPD, Wassmann P. 2005. Dynamics of transparent exopolymeric particles (TEP) production by Phaeocystis globosa under N- or P-limitation: a controlling factor of the retention/export balance? Harmful Algae 4: 895-914. Mari X, Rassoulzadegan F, Brussaard CPD. 2004. Role of TEP on the microbial food web structure: II. Influence on the ciliate community structure. Mar. Ecol. Prog. Ser. 279: 23-32. Brussaard CPD, Short SM, Frederickson CM, Suttle CA. 2004. Isolation and phylogenetic analysis of novel viruses infecting the phytoplankter Phaeocystis globosa (Prymnesiophyceae). Appl. Environ. Microbiol. 70(6): 3700-3705. Brussaard CPD. 2004. Viral Control of Phytoplankton Populations - a Review. J. Euk. Microbiol. 51(2): 125-138. Brussaard CPD. 2004. Optimization of procedures for counting viruses by flow cytometry. Appl. Environ. Microbiol. 70(3): 1506-1513. Brussaard CPD, Noordeloos AAM., Sandaa R-A, Heldal M, Bratbak G. 2004. Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla. Virology 319: 280-291. Thyrhaug R, Larsen A, Brussaard C, Bratbak G. 2002. Cell cycle dependent virus production in marine phytoplankton. Journal of Phycology 38: 338-343. Riegman R, Van Bleijswijk JDL, Brussaard CPD. 2002. The use of dissolved esterase activity as a tracer of phytoplankton lysis. Limnology and Oceanography 47(3): 916-930 Brussaard CPD, Marie D, Thyrhaug R, Bratbak G. 2001. Flow cytometric analysis of phytoplankton viability following viral infection. Aquatic Microbial Ecology 26(2): 157-166. Castberg T, Larsen A, Sandaa R-A, Brussaard CPD, Egge J, Heldal M, Paulino A, Thyrhaug R, Van Hannen E, Bratbak G. 2001. Microbial population dynamics and diversity during a bloom of the marine coccolithophorid Emiliania huxleyi (Haptophyta). Marine Ecology Progress Series 221: 39-46. Larsen A, Castberg T, Sandaa R-A, Brussaard CPD, Egge J, Heldal M, Paulino A, Thyrhaug R, Van Hannen E, Bratbak G. 2001. Population dynamics and diversity of phytoplankton, bacteria and virus in a seawater enclosure. Marine Ecology Progress Series 221: 47-57.