Molecular Biology Laboratory
The Molecular Biology laboratory facilitates DNA and RNA genetic approaches to study marine organisms. Scientists from NIOZ as well as from other institutions and companies are welcome to cooperate or to join proposals.
We may develop diagnostic tools for specific research questions and offer assistance and advice how to:
- sample for molecular biology analyses;
- process samples for your specific research;
- analyse sequences, (meta) genomes and transcriptomes;
- analyse the results statistically
Facilities
For applying our techniques we have several laboratories and rooms available, such as:
- Clean lab for preparing reagents
- DNA extraction lab
- RNA extraction lab
- Cycler room
- Post-PCR and electrophoresis laboratory
- Cloning room of class ML-II
- Computer room
Current research topics
Which organisms survive the treatment of ballast water
short tekst & picture
Quantification of viruses of eukaryote algae and cyanobacteria
info & picture
Nitrification in sponges by bacteria and archaea
info & picture
Aerobic and anaerobic ammonium oxidation in sediments of the North Sea
info & picture
Expression of genes related to calcification in corals
info & picture
Identification of bivalve larvae: monitoring of six bivalve species in the Wadden Sea
info & picture
Identification of larvae of Lutraria lutraria and Spisula subtruncata in the North Sea
info & picture
Sexing of birds
info & picture
Population genetics of the bivalve Scrobicularia plana
info & picture
Population genetics of the bivalve Loripes lacteus
info & picture
Cryptic species of Arenicola marina
info & picture
Genetic diversity of Nanochloropsis in relation to biomarker studies
info & picture
Loripes lacteus
Tropische sponzen met nitrificerende bacterien
Kleurrijke nonnetjes
Protocols and Methodology
DNA & RNA extraction
tekst
DGGE and sequencing
Oh Gerard je moest eens weten....
Cloning
text cloning
Microsatellite analysis
text microsatellite analysis
Handling sequencing data
How to
Recent publications
Selected publications
Cardoso JFMF, JDL van Bleijswijk, H Witte, FC van Duyl (2013) Diversity and abundance of ammonia oxidizing archaea and bacteria in tropical and cold-water coral reef sponges. Aquatic Microbial Ecology (in press)
Philippart CJM, A Amaral, R Asmus, J van Bleijswijk, J Bremner, F Buchholz, M Cabanellas-Reboredo, D Catarino, A Cattrijsse, F Charles, T Comtet, A Cunha, S Deudero, J-C Duchêne, S Fraschetti, F Gentil, A Gittenberger, JS Gray, K Guizien, JM Gonçalves, G Guarnieri, I Hendriks, B Hussel, R Pinheiro Vieira, B Reijnen, I Sampaio, E Serrao, I Sousa Pinto, E Thiebaut, F Viard, AF Zuur (2012) Spatial synchronies in the seasonal occurrence of larvae of oysters (Crassostrea gigas) and mussels (Mytilus edulis/galloprovincialis) in European coastal waters. Estuarine, Coastal and Shelf Science 108: 52-63
Stefan Schouten S, A Pitcher, EC Hopmans, L Villanueva, J van Bleijswijk, JS Sinninghe Damsté (2012) Intact polar and core glycerol dibiphytanyl glycerol tetraether lipids in the Arabian Sea oxygen minimum zone: I. Selective preservation and degradation in the water column and consequences for the TEX86. Geochimica et Cosmochimica Acta 98: 228-243
Luttikhuizen PC, A Bol, H Witte, J van Bleijswijk, O Haddrath, AJ Baker, T Piersma, J Reneerkens (2011) Novel and cross-species microsatellite markers for parentage analysis in sanderling Calidris alba. Journal of Ornithology DOI 10.1007/s10336-011-0681-6
Rampen SW, S Schouten, EC Hopmans, B Abbas, AAM Noordeloos, JDL van Bleijswijk, JAJ Geenevasen, JS Sinninghe Damsté (2009) Diatoms as a source for 4-desmethyl-23,24-dimethyl steroids in sediments and petroleum. Geochimica et Cosmochimica Acta 73(2):377-387
Weijers JWH, E Panoto, J van Bleijswijk, S Schouten, WIC Rijpstra, M Balk, AJM Stams, JS Sinninghe Damsté (2009) Constraints on the Biological Source(s) of the Orphan Branched Tetraether Membrane Lipids. Geomicrobiology Journal 26(6):402-414
Luttikhuizen PC, J Campos, J van Bleijswijk, KT Peijnenburg, HW van der Veer (2008) Phylogeography of the common shrimp, Crangon crangon (L.) across its distribution range. Molecular Phylogenetics and Evolution 46:1015–1030
Frade PR, F de Jongh, F Vermeulen, J van Bleijswijk, RPM Bak (2008) Variation in symbiont distribution between closely related coral species over large depth ranges. Molecular Ecology 17:691-703
Coolen, MJL, B Abbas, J van Bleijswijk, EC Hopmans, MMM Kuypers, SG Wakeham, JS Sinninghe Damsté (2007).Putative ammonia-oxidizing Crenarchaeota thrive in suboxic waters of the Black Sea: A basin-wide ecological study using 16S ribosomal and functional genes and membrane lipids. Environmental Microbiology 9(4):1001-1016
Wuchter C, B Abbas, MJL Coolen, L Herfort, J van Bleijswijk, P Timmers, M Strous, E Teira, GJ Herndl, JJ Middelburg, S Schouten, JS Sinninghe Damste (2006) Archaeal nitrification in the ocean. Proc. Natl. Acad. Sci. USA 103:12317–12322
